The challenge of locating targets with minimal DNA and RNA copies is a major disadvantage of using in situ hybridization techniques.
In situ hybridization's goals are to find out whether certain DNA or RNA sequences are present or absent and to localise these sequences to certain cells or chromosomal locations (Rautenstraub and Liehr, 2002). Utilizing a characteristic of nucleic acids, certain sequences are recognised within cells (i.e., their ability to specifically anneal to each other to form hybrids).
This method can be applied to combinations of RNA and DNA as well as two complementary strands of DNA. Hybrids of synthetic and natural nucleic acids are also conceivable. The hybridised probe is visible after a tagged probe is annealed to compatible sequences in fixed cells or tissue. The annealed hybrids can be identified by when one of the two strands is labelled.
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